Animal Cell Technology: Developments, Processes and Products by R. E. Spier

By R. E. Spier

This booklet provides a cutting-edge record at the box of animal mobilephone know-how, a resource of reference for all these excited by the creation and use of animal mobile items.

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Cell line 22H11 was originally selected in 20uM MSX and contains 5 copies of the heterologous gene (,,unamplifiedM gs-CHO), whereas cell line 32A2 was subsequently selected using 200uM MSX and contains approximately 200 heterologous gene copies per cell ("amplified" gs-CHO). Cell line 22H11 was passaged in glutamine- free medium without MSX selection whereas cell line 32A2 was passaged in glutamine-free medium with MSX selection. Figure la shows that the unamplified 22H11 gs-CHO cell line has a stable harvest titre in extended culture.

1988, 8, 1036-1044. E. H. Stage-specific transformation of murine B lineage cells by ras and mvc. Oncoaene, 1989, 4, 1425-1432. 25 ESTABLISHMENT OF DERMAL PAPILLA CELL LINES BY RETROVIRUS-MEDIATED TRANSFER OF ONCOGENES Amanda J. Stones, Wendy Filsell and Susan A. Bayley Unilever Research, Colworth House, Sharnbrook, Bedford, MK44 1LQ, UK ABSTRACT Passage 1 rat dermal papilla cells were transfected with immortalizing oncogenes and cell lines were established, which were shown to contain and express these genes (1).

The expression level of mRNA of v-myb was examined on BHK-21 cells and v-myb transfected BHK-21 cells. No expression of myb mRNA was detected on BHK-21 cells. About 10 copies of v-myb mRNA was expressed in a v-myb transfected BHK-21 cell. The productivities of highly productive cells generated by oncogenetransfection were very unstable. Highly productive cells tend to lower their productivities to several times higher level than control during subculture. DISCUSSION Nuclear oncogenes such as myc, myb, jun and fos are known to produce DNA binding proteins and activate specific promoters.

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